Bienzymatic Cascade Combining a Peroxygenase with an Oxidase for the Synthesis of Aromatic Aldehydes from Benzyl Alcohols

Aromatic aldehydes are important aromatic compounds for the flavour and fragrance industry. In this study, a parallel cascade combining aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) unspecific peroxygenase basidiomycete Agrocybe aegerita (AaeUPO) to convert primary alcohols into high-value is proposed. Key influencing factors in process of enzyme catalysis, such as dosage, pH temperature, were investigated. The universality PeAAOx coupled with AaeUPO catalysis synthesis aldehyde was evaluated. partially optimised system (comprising 30 μM PeAAOx, 2 at 7 40 °C) up 84% conversion 50 mM veratryl achieved self-sufficient aerobic reaction. Promising turnover numbers 2800 21,000 AaeUPO, respectively, point towards practical applicability.